Presentations from our Laboratory
Presentations from our Laboratory
Our laboratory has produced several presentations including the following (#denotes medical student, *undergraduates, §graduate students, †indicates invited presentations):
LA Ramos*, WJ Stark*, CG Kubista*, TA Whitaker*, J Meredith, F Cioffi, LM Cernick, BC Goess and SE Fiester. Modified siderophore as an effective inhibitor of Acinetobacter baumannii and Pseudomonas aeruginosa growth, Eagle X, April 2025.
Y Verma#, AT Sadek#, EM Wheibe#, HA Melzter#, K Connolly#, RF Relich, LA Actis, MS Ramírez, SE Fiester and JT Grier. Macrophage transcriptional responses to intracellular infection by necrotizing fasciitis-causing Acinetobacter baumannii and type strains, ASM Microbe 2025, June 2025.
AT Sadek#, Y Verma#, A Shidhaye#, N Bhatnagar*, HA Melzter#, E Wheibe#, K Connolly#, C Liu*, CR Gutiérrez*, BA Arivett§, RF Relich, LA Actis, SE Fiester, MS Ramírez and JT Grier. Macrophage cellular and transcriptional responses to infection with necrotizing fasciitis-causing multidrug-resistant isolates of Acinetobacter baumannii, Immunology 2025 The American Association of Immunologists Annual Meeting, May 2025.
†SE Fiester. Acinetobacter baumannii infection and novel treatments, Florida Gulf Coast University, February 2025.
C Evins, S Fiester, C Sutton, S Withers, JT Grier and CM Schammel. Community-acquired, extended-spectrum β-lactamase-producing and extensively drug-resistant Escherichia coli in a 28-year-old pyelonephritis patient lacking risk factors, Society of General Internal Medicine 2024 Southern Regional Meeting, February 2024.
AT Sadek#, EM Wheibe#, K Connolly#, C Liu*, CR Gutiérrez*, BA Arivett, RF Relich, LA Actis, S Fiester, MS Ramírez and JT Grier. Resistance properties and host immune cell entry of necrotizing fasciitis Acinetobacter baumannii isolates, ASM Microbe 2023, June 2023.
MR Ryan#, MC Stroud*, ZM Buchanan*, AR Rojas*, MP Knapp#, A Okinaga*, TO Connell, SE Fiester, S Arce and JT Grier. The immunomodulatory effects of E. coli heat-labile enterotoxins on the respiratory immune response, Immunology 2023 The American Association of Immunologists Annual Meeting, May 2023.
E Wheibe#, K Connolly#, RF Relich, SE Fiester and JT Grier. Investigation of drug-resistant Acinetobacter baumannii virulence factors implicated in intracellular survival within differentiated THP-1 cells, American Society for Clinical Pathology, September 2022.
MC Stroud*, AR Rojas*, MP Knapp#, A Okinaga*, TD Connell, SE Fiester, S Arce and JT Grier. The effects of type II heat-labile enterotoxins on human respiratory-specific immune responses, ASM Microbe, June 2022.
M Muqri*, K McCoy*, A Okinaga#, SE Fiester and JT Grier. Investigating the Antiviral and Antibacterial Response in CRISPR Cas9-Mediated Knockdown of Interferon Induced Protein 35 (IFI35) in a Human Lung Epithelial Cell Line, 2022 Spring Meeting of the South Carolina Branch of the American Society for Microbiology, April 2022.
-First Place in Undergraduate Category.
E Wheibe#, K Connolly#, R Relich, S Fiester and JT Grier. Investigation of drug-resistant Acinetobacter baumannii virulence factors implicated in intraceluular survival within differentiated THP-1 cells, Mid-Atlantic Microbial Pathogenesis Meeting, February 2022.
MC Stroud*, AR Rojas*, MP Knapp#, A Okinaga*, TD Connell, SE Fiester, S Arce and JT Grier. The effects of type II heat-labile enterotoxins on human respiratory-specific immune responses, Mid-Atlantic Microbial Pathogenesis Meeting, February 2022.
E Wheibe#, K Connolly#, RF Relich, SE Fiester and JT Grier. Investigation of drug-resistant Acinetobacter baumannii virulence factors implicated in intracellular survival within differentiated THP-1 Cells, Eastern-Atlantic Student Research Forum, January 2022.
K McCoy*, A Okinaga*, M Muqri*, SE Fiester and JT Grier. Investigating the antiviral and antibacterial response in CRISPR-Cas9 mediated knockdown of interferon induced protein 35 (IFI35) in a human lung epithelial cell line, Southeast Regional IDeA Meeting, November 2021.
MH Hyche*, CMG Schammel, M Devane, S Fiester, M Call, P Kent and J Knight. Tropheryma whipplei manifesting in the CNS: case report and comprehensive literature review, 2021 Fall Meeting of the South Carolina Branch of the American Society for Microbiology, October 2021.
AR Rojas*, MC Stroud*, MP Knapp#, A Okinaga*, TD Connell, SE Fiester, S Arce and JT Grier. Investigating the effects of type II heat-labile enterotoxins on human respiratory immune responses, 2021 Fall Meeting of the South Carolina Branch of the American Society for Microbiology, October 2021.
E Wheibe#, K Connolly#, SE Fiester and JT Grier. Examination of drug-resistant Acinetobacter baumannii response to phagocytosis by monocyte-derived macrophages, 2021 Fall Meeting of the South Carolina Branch of the American Society for Microbiology, October 2021.
-Second Place in Medical Student Category
AR Rojas*, MC Stroud*, MP Knapp#, A Okinaga*, TD Connell, SE Fiester, S Arce and JT Grier. Investigating the effects of type II heat-labile enterotoxins on human respiratory immune responses, 2021 Prisma Health Research Showcase, October 2021.
E Wheibe#, K Connolly#, SE Fiester and JT Grier. Examination of drug-resistant Acinetobacter baumannii response to phagocytosis by monocyte-derived macrophages, 2021 Prisma Health Research Showcase, October 2021.
K McCoy*, A Okinaga*, M Muqri*, SE Fiester and JT Grier. Investigating the antiviral and antibacterial response in CRISPR-Cas9 mediated knockdown of interferon induced protein 35 (IFI35) in a human lung epithelial cell line, Biomedical Sciences Student Research Symposium, July 2021.
M Stroud*, A Rojas*, MP Knapp#, A Okinaga*, SE Fiester, S Arce, and JT Grier. Investigating the effects of heat-labile enterotoxins in a respiratory immunization model, Biomedical Sciences Student Research Symposium, July 2021.
A Rojas*, M Stroud*, MP Knapp#, A Okinaga*, SE Fiester, S Arce and JT Grier. Investigating the effects of heat-labile enterotoxins on cytokine secretion by human respiratory epithelial cells, Biomedical Sciences Student Research Symposium, July 2021.
C Liu*, CR Gutiérrez*, BA Arivett, RF Relich, JT Grier, LA Actis, SE Fiester and M Ramírez. Changes in resistance properties of extensively drug-resistant Acinetobacter baumannii over the course of a lethal infection, World Microbe Forum, June 2021.
†SE Fiester. Conotton Valley Union Local Schools High School Graduation Commencement Address, Bowerston, OH, May 2021.
C Hathaway#, CMG Schammel and SE Fiester. Aspergillus otomycosis leading to tympanic membrane rupture in a young immunocompetent woman, Discover USC, Columbia, SC, April 2021.
MH Hyche*, CMG Schammel, M Devane, S Fiester, M Call, P Kent, J Knight. Tropheryma whipplei manifesting in the CNS: case report and comprehensive literature review, Discover USC, Columbia, SC, April 2021.
-First Place in Case Reports/Small Series/Learning Innovation Category
K McCain*, JT Grier and SE Fiester. Elucidation of heme-b utilization mechanisms by the human nosocomial pathogen Acinetobacter baumannii, Discover USC, Columbia, SC, April 2021.
C Hathaway#, CMG Schammel and SE Fiester. Aspergillus otomycosis in a young immunocompetent woman, SC Upstate Research Symposium (SCURS), Spartanburg, SC, April 2021.
-Presented as a conference proceeding
†E Hogue*, L Bracken*, SE Fiester and JT Grier. Development of Models for Study of Host-Pathogen Interactions During Human Acinetobacter baumannii infection via Novel Dual RNA-sequencing Technology, 2021 SC-INBRE Science Symposium, Columbia, SC, January 2021.
L Bracken*, E Hogue*, SE Fiester and JT Grier. Exploration of the host-pathogen interactions of Acinetobacter baumannii and human lung epithelial cells with novel dual RNA-sequencing technology, 2020 Fall Meeting of the South Carolina Branch of the American Society for Microbiology, Greenville, SC, October 2020.
JA Kohrt*, M Young*, N Nawar*, DO Boldt*, J Valbuena*, SE Fiester, A Tartaro and JT Grier. Applications of R and Shiny for interactive visualizations of host-pathogen interactions in dual RNA-seq data, 2020 Fall Meeting of the South Carolina Branch of the American Society for Microbiology, Greenville, SC, October 2020.
-This presentation won “Second Place Undergraduate Category.”
MA Knapp#, TA Johnson#, MK Ritter#, RO Rainer, SE Fiester, JT Grier, TD Connell and S Arce. Differential binding of E. coli enterotoxins LT-IIa, LT-IIb and LT-IIc to human B and T cell subsets identifies a potential use as adjuvants in cancer immunotherapy, American Association for Cancer Research Virtual Special Conference on Tumor Immunology and Immunotherapy, Philadelphia, PA, October 2020.
DC Lee#, SE Fiester, LA Madeline, G Dukes, JW Fulcher, ME Ward, CMG Schammel and RK Hakimi. Fatal granulomatous amebic encephalitis in Upstate South Carolina, 2020 Fall Meeting of the South Carolina Branch of the American Society for Microbiology, Greenville, SC, October 2020.
K McCain*, JT Grier and SE Fiester. Elucidation of heme-b utilization mechanisms by the human nosocomial pathogen Acinetobacter baumannii, 2nd Annual Biomedical Sciences Student Research Virtual Symposium, Greenville, SC, July 2020.
L Bracken*, E Hogue*, S Fiester and JT Grier. Dual RNA-Seq of A. baumannii with human host, 2nd Annual Biomedical Sciences Student Research Virtual Symposium, Greenville, SC, July 2020.
C Liu*, CR Gutiérrez*, BA Arivett, RF Relich, JT Grier, LA Actis, SE Fiester and MS Ramírez. Changes in antimicrobial resistance properties of extensively drug-resistant Acinetobacter over the course of a lethal infection, American Society for Microbiology Microbe 2020, Chicago, IL, June 2020.
-Abstract accepted, conference cancelled due to SARS-CoV-2 pandemic
GM Traglia*, J Fernandez*, JE Reddic, JF Meredith, SE Fiester and M Ramirez. Genomic diversity of Acinetobacter clinical isolates from a large southeastern United States hospital system, American Society for Microbiology Microbe 2020, Chicago, IL, June 2020.
-Abstract accepted, conference cancelled due to SARS-CoV-2 pandemic
B. Tennie§, SE Fiester, JR Meredith, JW Kelly, SE Williams, JE Reddic, SD Lee#, DC Lee#, JA Bigner# and MS Nolan. Evaluating the clinical epidemiology of Klebsiella infections in the Upstate region of South Carolina, Prisma Health, Health Sciences Center Research Showcase, Greenville, SC, April 2020.
-Abstract accepted, conference cancelled due to SARS-CoV-2 pandemic
†DC Lee#, SE Fiester, LA Madeline, JW Fulcher, ME Ward, CMG Schammel and RK Hakimi. Acanthamoeba spp. and Balamuthia mandrillaris leading to fatal granulomatous amebic encephalitis, 4th Annual Harriet and Jerry Dempsey Research Conference, Greenville, SC, March 2020.
-Abstract accepted, conference cancelled due to SARS-CoV-2 pandemic
†DC Lee#, SE Fiester, LA Madeline, JW Fulcher, ME Ward, CMG Schammel and RK Hakimi. Acanthamoeba spp. and Balamuthia mandrillaris leading to fatal granulomatous amebic encephalitis, 43rd Annual Meeting of the American Society of Neuroimaging (ASN), Atlanta, GA, March 2020.
-DC Lee awarded $1000 from Prisma Health Department of Internal Medicine for travel
†JT Grier. Dual RNA-seq for the analysis of host-pathogen interactions in a model of A. baumannii infection, National Big Data Health Science Conference 2020, Columbia, SC, February 2020.
-Presentation of 2019 BIPP grant awarded to JT Grier and SE Fiester as Co-PIs.
L Bracken*, E Hogue*, S Fiester and JT Grier. Dual RNA-Seq of A. baumannii with human host, USCSOMG Biomedical Sciences Summer Research Symposium, Greenville, SC, July 2019.
BA Arivett§, JT Grier, JA Bigner#, EJ Ohneck§, ML Metz*, CR Wood§, RF Relich, LA Actis and SE Fiester. Increased twitching motility, biofilm formation and virulence of Acinetobacter baumannii associated with necrotizing fasciitis, ASM Microbe 2019, San Francisco, CA, June 2019.
†SE Fiester, BA Arivett§, AC Beckett§, BR Wagner#, EJ Ohneck§, RE Schmidt* and LA Actis. Rapid Fire Talk (Outside the Box Therapeutics): Hexadecylphosphocholine reduces Acinetobacter baumannii virulence, ASM Microbe 2018, Atlanta, GA, June 2018.
SE Fiester, BA Arivett§, AC Beckett§, BR Wagner#, EJ Ohneck§, RE Schmidt* and LA Actis. Hexadecylphosphocholine reduces Acinetobacter baumannii virulence, ASM Microbe 2018, Atlanta, GA, June 2018.
†S Arce, A Blenda, R Chosed, S Fiester and W Roudebush. Introduction to the Biomedical Researchers at USCSOM-Greenville, Greenville Health System Research Division Advisory Group quarterly meeting, Greenville, SC, February 2018.
BA Arivett§, T Gerke, SE Fiester and A Farone. Use of synchrotron radiation to investigate heme-like gallium protoporphyrin IX in Acinetobacter baumannii, Kentucky-Tennessee Branch ASM Fall 2017 Meeting, Cookeville, TN, November 2017.
-This presentation won “Best Graduate Poster.”
EJ Ohneck§, BA Arivett§, SE Fiester, CR Wood§, ML Metz*, GM Simeone* and LA Actis. Mucin acts as a signal for the differential expression of cellular processes and virulence factors in Acinetobacter baumannii, 14th Annual Cell, Molecular and Structural Biology (CMSB) Symposium, Miami University, Oxford, OH, June 2016.
EJ Ohneck§, BA Arivett§, SE Fiester, GM Simeone*, MM Metz* and LA Actis. Investigating the role of the Acinetobacter baumannii two-component system BfmRS in sensing and responding to mucin, 22nd Annual Midwest Microbial Pathogenesis Conference, Indianapolis, IN, August 2015.
CM Harding§, MA Nasr, RL Kinsella, BS Weber, SE Fiester, LA Actis, EN Tracy, RS Munson and MF Feldman. Acinetobacter strains carry two functional oligosaccharyltransferases, one devoted exclusively to type IV pili and one devoted to O-glycosylation of multiple proteins, Acinetobacter 2015 10th International Symposium on the Biology of Acinetobacter, Athens, Greece, June 2015.
CR Wood§, BA Arivett§, SE Fiester and LA Actis. Role of a novel type I pilus in Acinetobacter baumannii ATCC 17978, Ohio Branch of the American Society for Microbiology Annual Meeting, Lorain, OH, April 2015.
A Tomaras, J Crandon, C McPherson, WF Penwell§, SE Fiester, BA Arivett§, LA Actis and D Nicolau. Lack of correlation between in vitro activity and in vivo efficacy of MB-1, a siderophore-β-lactam conjugate, against multiple strains of Acinetobacter baumannii, ICAAC 2014, Washington, DC, September 2014.
SC Geiger*, BA Arivett§, SE Fiester and LA Actis. Determining the antimicrobial mode of action of GaPPIX on Acinetobacter baumannii, 12th Annual Cell, Molecular and Structural Biology (CMSB) Symposium, Miami University, Oxford, OH, May 2014.
BA Arivett§, WF Penwell§, CM Kaufman, RF Relich, SE Fiester and LA Actis. Gallium protoporphyrin IX inhibits growth of multidrug-resistant Acinetobacter baumannii, 114th General Meeting of the American Society for Microbiology, Boston, MA, May 2014.
EJ Ohneck§, SE Fiester, DL Zimbler§, PN Rather and LA Actis. Characterization of the BfmRS two-component regulatory system in Acinetobacter clinical strains, 114th General Meeting of the American Society for Microbiology, Boston, MA, May 2014.
SE Fiester, CC Nwugo, WF Penwell§, J Neary, AC Beckett*, BA Arivett§, PL Connerly, SM Menke, AP Tomaras and LA Actis. Role of the carboxyl terminus of SecA in iron acquisition, protein translocation and virulence in the bacterial pathogen Acinetobacter baumannii, 114th General Meeting of the American Society for Microbiology, Boston, MA, May 2014.
SE Fiester, CC Nwugo, WF Penwell§, JM Neary, AC Beckett*, ML Metz*, BA Arivett§, PM Connerly, SM Menke, AP Tomaras and LA Actis. Role of the carboxyl terminus of SecA in iron acquisition, protein translocation and virulence of the bacterial pathogen Acinetobacter baumannii, Ohio Branch of the American Society for Microbiology Annual Meeting, Columbus, OH, April 2014.
SE Fiester, BA Arivett§, AC Beckett*, RE Schmidt*, ML Metz*, MV Carrier* and LA Actis. Iron-regulated cytolytic activity plays a role in the virulence of the human pathogen Acinetobacter baumannii, Ohio Branch of the American Society for Microbiology Annual Meeting, Columbus, OH, April 2014.
GM Simeone*, EJ Ohneck§, BA Arivett§, SE Fiester and LA Actis. Acinetobacter baumannii sensor kinase BfmS is involved in sensing pulmonary surfactant components, Ohio Branch of the American Society for Microbiology Annual Meeting, Columbus, OH, April 2014.
D Hariraju§, T Ticak§, M Bayron Arcelay*, SE Fiester and DJ Ferguson Jr. Isolation and characterization of novel strains of quaternary amine utilizing archaea from estuarine sediment, Ohio Branch of the American Society for Microbiology Annual Meeting, Columbus, OH, April 2014.
SC Geiger*, BA Arivett§, SE Fiester and LA Actis. Determining the antimicrobial mode of action of GaPPIX on Acinetobacter baumannii, Ohio Branch of the American Society for Microbiology Annual Meeting, Columbus, OH, April 2014.
SE Fiester, CC Nwugo, J Neary, WF Penwell§, BA Arivett§, AC Beckett*, S Menke, AP Tomaras and LA Actis. Role of the C-terminus of SecA in iron acquisition and protein translocation by Acinetobacter baumannii ATCC 19606T, Cell, Molecular and Structural Biology (CMSB) Symposium, Miami University, Oxford, OH, May 2013.
ML Metz*, BA Arivett§, AC Beckett*, MV Carrier*, WF Penwell§, SE Fiester and LA Actis. Phosphatidylcholine-specific phospholipase C activity is responsible for the hemolytic activity of A. baumannii, Cell, Molecular and Structural Biology (CMSB) Symposium, Miami University, Oxford, OH, May 2013.
EJ Ohneck§, DL Zimbler§, SE Fiester, WF Penwell§ and LA Actis. Characterization of the sensor kinase BfmS in multiple Acinetobacter baumannii strains, Cell, Molecular and Structural Biology (CMSB) Symposium, Miami University, Oxford, OH, May 2013.
SE Fiester, CC Nwugo, J Neary, WF Penwell§, BA Arivett§, AC Beckett*, S Menke, AP Tomaras and LA Actis. Role of the C-terminus of SecA in iron acquisition and protein translocation by Acinetobacter baumannii ATCC 19606T, Ohio Branch of the American Society for Microbiology Annual Meeting, Ashland, OH, April 2013.
ML Metz*, BA Arivett§, AC Beckett*, MV Carrier*, WF Penwell§, SE Fiester and LA Actis. Phosphatidylcholine-specific phospholipase C activity is responsible for the hemolytic activity of A. baumannii, Ohio Branch of the American Society for Microbiology Annual Meeting, Ashland, OH April 2013.
EJ Ohneck§, DL Zimbler§, SE Fiester, WF Penwell§ and LA Actis. Characterization of the sensor kinase BfmS in multiple Acinetobacter baumannii strains, Ohio Branch of the American Society for Microbiology Annual Meeting, Ashland, OH, April 2013.
ML Metz*, BA Arivett§, AC Beckett*, MV Carrier*, WF Penwell§, SE Fiester and LA Actis. Examining hemolytic activity in Acinetobacter baumannii, Undergraduate Research Forum, Miami University, Oxford, OH, April 2013.
MK Sellin-Jeffries, BA Arivett§, SE Fiester, DD Coffey*, LM Thornton*, AW Smith*, LA Actis, JT Oris. Development of two small fish species, Pimephales promelas and Cyprinodon variegates, as model organisms for immunotoxicity, Society of Toxicology and Environmental Chemistry North America 33rd Annual Meeting, Long Beach, CA, November 2012.
SE Fiester, BA Arivett§, MV Carrier*, AC Beckett* and LA Actis. Hemolytic activity of Acinetobacter baumannii, 19th Annual Midwest Microbial Pathogenesis Conference, Milwaukee, WI, September 2012.
BA Arivett§, SE Fiester, WF Penwell§, DL Zimbler§ and LA Actis. Exploiting an iron vein: Acinetobacter baumannii’s acquisition of iron from erythrocytes, 19th Annual Midwest Microbial Pathogenesis Conference, Milwaukee, WI, September 2012.
EJ Ohneck§, DL Zimbler§, SE Fiester, BA Arivett§ and LA Actis. Acinetobacter baumannii two-component regulatory system bfmRS is involved in biofilm formation on both biotic and abiotic surfaces, 19th Annual Midwest Microbial Pathogenesis Conference, Milwaukee, WI, September 2012.
EJ Ohneck§, SE Fiester, DL Zimbler§ and LA Actis. Acinetobacter baumannii sensor kinase BfmS is involved in the regulation of motility and biofilm formation, 20th Annual Midwest Microbial Pathogenesis Conference, Columbus, OH, August 2012.
WF Penwell§, DL Zimbler§, AC Beckett*, AM Richards*, BA Arivett§, EJ Ohneck§, SE Fiester, DV Zurawski and LA Actis. Variability of virulence factors among Acinetobacter baumannii isolates obtained from wounded military personnel, 20th Annual Midwest Microbial Pathogenesis Conference, Columbus, OH, August 2012.
CM Harding§, EN Tracy, MD Carruthers, SE Fiester, LA Actis and RS Munson, Jr. Demonstration of type IV pili production in Acinetobacter baumannii and their role in natural transformation, twitching motility and biofilm formation, 20th Annual Midwest Microbial Pathogenesis Conference, Columbus, OH, August 2012.
AC Beckett*, MV Carrier*, BA Arivett§, SE Fiester and LA Actis. Hemolytic activity of Acinetobacter baumannii, Cell, Molecular and Structural Biology (CMSB) Symposium, Miami University, Oxford, OH, June 2012.
MV Carrier*, BA Arivett§, SE Fiester and LA Actis. Analysis of uncharacterized hemolytic activity expressed by Acinetobacter baumannii, Ohio Branch of the American Society for Microbiology Annual Meeting, Mason, OH, April 2012.
†SE Fiester. Characterization of optically active biopolymers, Kent State University Department of Biological Sciences’ Seminar Series, Kent, OH, April 2011.
†SE Fiester. Characterization of optically active bacterial and viral proteins, Ohio Branch American Society for Microbiology, Ohio University, Athens, OH, April 2011.
SE Fiester. Detection of immune complexes using chiral nematic chitin fibrils, 26th Annual Graduate Research Symposium, Kent, OH, April 2011.
†SE Fiester. Optical properties of a viral protein, Kent State Liquid Crystal Institute Liquid Crystal Day, Kent, OH, October 2010.
SE Fiester, A Jákli and CJ Woolverton. Liquid crystalline properties of g8p, the major coat protein of fd bacteriophage, 84th Colloid on Surface Science Symposium, Akron, OH, June 2010.
SE Fiester and CJ Woolverton. Formulation of chitin as a liquid crystal biosensor, Ohio Branch of the American Society for Microbiology Annual Meeting, Mason, OH, April 2010.
TT Meilander§, SL Helfinstine§, SE Fiester and CJ Woolverton. Hands-on biosafety level 3 workshop improves knowledge and skill in laboratory personnel, American Society for Microbiology 107th General Meeting, Toronto, Canada, May 2007.
†SE Fiester. American Society for Clinical Laboratory Science (OH), Toledo, OH, 2006.